• Combating antibiotic resistance: An approach to discover antimicrobial compounds 

      Wigand, Johannes; Winje, Endre (Mastergradsoppgave; Master thesis, 2022-05-01)
      Objective: This study aims to test a cost-effective approach for discovery of novel molecular compounds with antimicrobial characteristics. Materials and methods: Thirteen randomly picked objects were swabbed in the area in and around Liverpool. They were further cultivated on BHI-agar plates, and then spread on top of several clinically relevant pathogenic indicator strains including C. albicans, ...
    • Functional screening of a human saliva metagenomic DNA reveal novel resistance genes against sodium hypochlorite and chlorhexidine 

      Wigand, Johannes; Tansirichaiya, Supathep; Winje, Endre; Al-Haroni, Mohammed (Journal article; Tidsskriftartikkel; Peer reviewed; Mastergradsoppgave; Master thesis, 2021-12-09)
      Objective - Many sections of the health care system are facing a major challenge making infectious disease problematic to treat; antimicrobial resistance (AMR). Identification and surveillance of the resistome have been highlighted as one of the strategies to overcome the problem. This study aimed to screen for AMR genes in an oral microbiota, a complex microbial system continuously exposed to ...
    • Inverse PCR-based detection reveal novel mobile genetic elements and their associated genes in the human oral metagenome 

      Tansirichaiya, Supathep; Winje, Endre; Wigand, Johannes; Al-Haroni, Mohammed (Journal article; Tidsskriftartikkel; Peer reviewed, 2022-05-27)
      The human oral cavity is one of the hotspots harboring multiple mobile genetic elements (MGEs), which are segments of DNA that can move either within bacterial genomes or between bacterial cells that can facilitate the spreading of genetic materials, including antimicrobial resistance genes. It is, therefore, important to investigate genes associated with the MGEs as they have a high probability of ...